Diferentes métodos para la determinación de la actividad nitrato reductasa en tejidos de Lotus sp.

O. Borsani; H. Urrestarazú; P. Díaz

doi:10.31285/AGRO.01.1082

Differents methods to the determination of nitrate resductase activity in Lotus sp. tissues

Authors

O. Borsani Laboratorio de Bioquímica, Facultad de Agronomía. Av. Garzón 780. Montevideo, Uruguay
H. Urrestarazú Laboratorio de Bioquímica, Facultad de Agronomía. Av. Garzón 780. Montevideo, Uruguay
P. Díaz Laboratorio de Bioquímica, Facultad de Agronomía. Av. Garzón 780. Montevideo, Uruguay

DOI:

https://doi.org/10.31285/AGRO.01.1082

Keywords:

lotus. sp., nitrate reductase, in situ assay, in vitro assay, in vivo assay

Abstract

Nitrate reductase activity (NRA) from stem and leaves of Lotus corniculatus and Lotus tenuis was assayed in vivo, in situ and in vitro. There was a strong correlation comparing assays in vivo with and without added nitrate and between in situ and in vivo without added nitrate. The highest absolute values of NRA in all tissues assayed where obtained using the in vivo with added nitrate methodology. The advantages and disadvantages of the differents methods in the estimation of NRA for birdsfoot trefoil are discussed.

Downloads

Download data is not yet available.

Downloads

PDF (Español)

Published

1997-06-01

How to Cite

Borsani O, Urrestarazú H, Díaz P. Differents methods to the determination of nitrate resductase activity in Lotus sp. tissues. Agrocienc Urug [Internet]. 1997 Jun. 1 [cited 2024 Jul. 6];1(1):50-4. Available from: http://mail.revista.asocolderma.org.co/index.php/agrociencia/article/view/1082